What are the possible reason for no colonies to grow on agarose plate?

How does inefficient ligation result in no colony growth on agarose plate?

Answer: Possible Reasons - Low concentration of DNA fragments, reaction volume more than 10μl, unequal concentration of control and experiment for transformation.

Incorrect ligation and poor ligase activity result in no colony growth on agarose plate.

Explanation:

Low concentration of DNA will not allow colonies to grow on agarose plate. Reaction volume must be less than 10μl. Different concentration of control and experiment will also affect growth of colonies.

Inefficient ligation result in no colony growth on agarose plate. This could be due to incorrect ligation between blunt and sticky ends. T4 DNA ligase joins the ends of DNA. Ligase poor activity will not allow colonies to grow on agarose plate.