Describe the construction of a recombinant plasmid containing the gene for the green fluorescent protein (GFP) and the insertion of the plasmid into a bacterial cell by placing the steps in order

-ligate together the GFP gene and the plasmid to generate a recombinant plasmid-transform bacteria with the recombinant plasmid using electroporation

-use PCR to amplify the gene for GFP

-perform a restriction digestion of the GFP gene and the plasmid

-plate the bacterial cells, and screen for positive transformants

Question

Louis Davenport

Biology

1 November, 17:58

Describe the construction of a recombinant plasmid containing the gene for the green fluorescent protein (GFP) and the insertion of the plasmid into a bacterial cell by placing the steps in order

-ligate together the GFP gene and the plasmid to generate a recombinant plasmid-transform bacteria with the recombinant plasmid using electroporation

-use PCR to amplify the gene for GFP

-perform a restriction digestion of the GFP gene and the plasmid

-plate the bacterial cells, and screen for positive transformants

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Louie · Answer 1

-use PCR to amplify the gene for GFP

-perform a restriction digestion of the GFP gene and the plasmid

-ligate together the GFP gene and the plasmid to generate a recombinant plasmid

-transform bacteria with the recombinant plasmid using electroporation

-plate the bacterial cells, and screen for positive transformants