How many bands would you expect to see after completing DNA gel electrophoresis and staining the gel?

Question

Alberto Golden

Biology

15 March, 17:08

How many bands would you expect to see after completing DNA gel electrophoresis and staining the gel?

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Answers (2)

Know the Answer?

Dyer · Answer 1

Answer: The number of bands of DNA varies according to molecules of different sizes but ranges from 100bp to 25kb.

Explanation: Gel electrophoresis is a laboratory effective method of separating mixtures of DNA, RNA or proteins. Gel electrophoresis includes electrical field where molecules to be separated are pushed by electrical field through a gel of small pores. The molecules pass through the gel at speed rate inversely related to their length and DNA with small molecule travel a greater distance through the gel than that of a large DNA molecule.

Therefore the size of the molecules of DNA determines the number of bands to be detected.

Aarav Stanton · Answer 2

The number of bands visible after completing DNA gel electrophoresis and staining the gel depends on the amount of DNA use and the DNA fragments will separate according to their sizes.

Explanation:

Based on their size and charge, molecules will travel through the gel in different directions or at different speeds, allowing them to separate from one another. All DNA molecules have the same amount of charge per mass, because of this gel electrophoresis of DNA fragments separate them based on the size only.