Hershey and Chase grew some T2 phages in 32P-containing medium and then used that phage to infect a bacterial culture. After the infection they detached the phage heads from the bacteria using a blender. Then they centrifuged the culture to separate the bacterial pellet and the supernatant. What fraction will be the most radioactive?

Question

Anthony Bright

Chemistry

20 May, 00:11

Hershey and Chase grew some T2 phages in 32P-containing medium and then used that phage to infect a bacterial culture. After the infection they detached the phage heads from the bacteria using a blender. Then they centrifuged the culture to separate the bacterial pellet and the supernatant. What fraction will be the most radioactive?

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Answers (1)

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Alannah Cannon · Answer 1

The pellet fraction will be most radioactive because the heavy protein part of ghost will be present in the the pellet fraction.

Explanation:

supernatant can be defined as a liquid part that lies above the sediment of the centrifuge tube on the other hand pallet is a solid material present at the bottom end of the centrifuge tube.

As protein molecules in the given experiment is radio labelled that"s why the pellet fraction will be radio labeling part because the heavy protein molecules will be present in the pellet fraction.