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3 July, 00:09

The original concentration in a sample is 5.1 x 10^9 cfu/ml. which sample volume should yield a countable plate

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  1. 3 July, 00:27
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    The original concentration is 5.1 * 10⁹ CFU / ml

    In order to attain a countable plate, the number of CFU must be present in between 10 and 200 per ml.

    Let us take 0.1 ml and dilute it to 1 ml.

    This minimizes the concentration to 5.1 * 10⁹ * 10⁻¹ = 5.1 * 10⁸ CFU/ml

    In order to minimize the concentration in between 10 and 200, it can be reduced to 5.1 * 10¹

    The final concentration = 5.1 * 10¹ CFU/ml

    Initial concentration = 5.1 * 10⁹ CFU/ml

    Volume of sample with 5.1 * 10¹ CFU = 5.1 * 10¹ CFU * (1 ml / 5.1 * 10⁹ CFU)

    = 1.0 * 10⁻⁸ ml

    This is the volume to be taken to obtain countable value, 51 CFU.
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