Which chemicals are used during the Gram Stain procedure?

4 chemicals

Explanation:

There are mainly 4 steps of gram staining.

1 - crystal violet stain drained on the heat fixed slide.

2 - then wash the slide and use iodine.

3 - then wash the slide with water and use acetone as a decolorizer.

4 - then use safranine as a counter stain.

So there are a total of 4 chemicals used named crystal violet, iodine, acetone, and safranine.