Plasmids are used as vectors in both plant and bacterial recombinant DNA technology. However, there is a major difference in the fate of genes introduced into bacteria on most bacterial plasmids and into plants on Ti plasmids. What is this difference?

Question

Braelyn

Biology

1 April, 16:23

Plasmids are used as vectors in both plant and bacterial recombinant DNA technology. However, there is a major difference in the fate of genes introduced into bacteria on most bacterial plasmids and into plants on Ti plasmids. What is this difference?

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Answers (1)

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Madison · Answer 1

DNA carried on bacterial plasmids are generally not integrated into the genome. The DNA carried on Ti plasmids are usually integrated into the plant genome, meaning the genes are stably expressed

Explanation:

A single strand of the T-DNA from the Ti plasmid enters the plant cell through a transport channel and into the nucleus, where it is integrated into the plant's DNA. In contrast, the DNA introduced into bacteria on bacterial plasmids remains extra-chromosomal