Some of these steps are reversible and catalyzed by the same enzyme acting in either direction, glycolysis or gluconeogenesis. Which reaction steps are irreversible and require a different enzyme in gluconeogenesis than in glycolysis?

There are 3 irreversible steps in Gluconeogenesis that are catalyzed by different enzymes than in Glycolysis namely:

1) Conversion of pyruvate to phosphoenolpyruvate (PEP) catalyzed by pyruvate carboxylase.

2) Conversion of fructose-1,6-bisphosphate to fructose-1,6-phosphate catalyzed by fructose-1,6-phosphatase.

3) Conversion of glucose-6-phosphate to glucose by the enzyme glucose-6-phosphatase.