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27 August, 14:15

While performing the acid-fast stain procedure, you heat your fixed bacteria with carbolfuchsin for 5 minutes, let the slide cool, and then rinse with water. what is your next step?

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  1. 27 August, 14:34
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    In the acid-fast or Ziehl-Neelsen stain, following the referred washing, the slide should be covered in 1%-3% acid alcohol to decolourise until the smear turns into light pink. After that, wash well with clean water to stop the decolourisation.
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