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26 March, 18:45

Which is more efficient: blunt-end cloning or sticky-end cloning? Why?

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  1. 26 March, 18:46
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    The correct answer will be option - the sticky ends are more efficient than blunt ends.

    Explanation:

    The molecular cloning is the commonly used technology which uses restriction enzymes to make cuts in the DNA. Depending on the activity of these enzymes, they produce two types of ends: sticky ends and blunt ends.

    The sticky ends produce unpaired nucleotide overhangs whereas blunt end produces paired nucleotide. The advantage of the overhangs is that they find complementary overhangs to bind as quickly as possible and require less amount of DNA ligase to join nucleotide. This ensures that the sticky ends DNA binds in the right direction.

    Thus, the sticky ends are more efficient than blunt ends.
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