In some cases, recombinant DNA must be cloned before it can be inserted into a host. Which vectors can be used to clone recombinant DNA?

I believe these are the steps

1. Isolate DNA from the organism

2. Cut DNA with restriction enzymes

3. Insert DNA fragments into a "vector" (plasmid)

4. Introduce recombinant DNA into bacterial host cell (E. coli) for replication

5. Grow the host and reisolate recombinant DNA clones

**to distinguish host cells carrying plasmids with dna inserts, the vector should have a selectable marker gene - usually an antibiotic gene or an enzyme absent from host cell, when plated on this antibiotic it will be obvious to see host cells.