2.) If you wanted to express a large eukaryotic gene in a prokaryotic organism, what steps would be necessary? Describe your protocol below including the suitability of a vector for a large gene.

The eukaryotic genes of interest can be expressed in the prokaryotic genome or plasmid vector. The following are the steps used for the purpose of expression of eukaryotic genes in the prokaryotic genome.

1. Amplification of the target gene of interest from the eukaryotic genome by PCR or polymerase chain reaction.

2. The selection of the plasmid and digestion of the plasmid DNA with the restriction enzyme.

3. Ligation of the amplified DNA fragment and the plasmid.

4. Introduction of the ligated plasmid into the prokaryotic host organism.