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22 September, 19:13

In blue-white screening, what do blue colonies represent?

cells that have not taken up the plasmid vector

cells with recombinant plasmids containing a new insert

cells containing empty plasmid vectors

cells with a non-functional lacZ gene

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  1. 22 September, 19:34
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    The correct option is : cells containing empty plasmid vectors

    Explanation:

    The blue-white screen is a technique which involves the rapid identification of the recombinant bacteria in a vector-based molecular cloning experiment. In this method, a DNA ligated vector is inserted in a host cell which is viable for transformation and grown in presence of X-gal.

    The cells that are transformed with the vectors having the recombinant DNA produce the white colonies. Whereas, the cells transformed with empty vector i. e. the non-recombinant plasmids, produce the blue colonies.
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