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Dixie Ballard
Biology
9 July, 04:56
How does cloning work and work are the basics
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Duchess
9 July, 04:58
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How cloning works: In reproductive cloning, researchers remove a mature somatic cell, such as a skin cell, from an animal that they wish to copy. They then transfer the DNA of the donor animal's somatic cell into an egg cell, or oocyte, that has had its own DNA-containing nucleus removed ... This young animal is referred to as a clone.
Basics of cloning:
Isolation of target DNA fragments (often referred to as inserts)
Ligation of inserts into an appropriate cloning vector, creating recombinant molecules (e. g., plasmids)
Transformation of recombinant plasmids into bacteria or other suitable host for propagation.
Screening/selection of hosts containing the intended recombinant plasmid.
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