During purification of many cytosolic proteins, it is important to

include either dithiothreitol or b-mercaptoethanol in the

buffers. Why?

Question

Harrison

Biology

4 February, 07:14

During purification of many cytosolic proteins, it is important to

include either dithiothreitol or b-mercaptoethanol in the

buffers. Why?

+3

Answers (1)

Know the Answer?

Derick Hughes · Answer 1

To reduce thiol-groups of the protein

Explanation:

Dithiothreitol of b-mercaptoethanol act as reducing agents. Including them in a buffer would prevent oxidation of thiol-groups in amino acids of the protein and preventing its denaturation.

During purification of many cytosolic proteins, it is important toinclude either dithiothreitol or b-mercaptoethanol in thebuffers. Why?

During purification of many cytosolic proteins, it is important to

include either dithiothreitol or b-mercaptoethanol in the

buffers. Why?