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14 August, 10:02

Which of the following vectors holds the largest pieces of DNA?

a. plasmids

b. bacteriophage

c. YACs

d. PACs

e. cosmids

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Answers (1)
  1. 14 August, 10:04
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    c. YACs

    Explanation:

    YACs, the Yeast artificial chromosomes are the high capacity vectors designed to carry the eukaryotic genes and carry the insert of 200-2000 kb.

    YACs carry origin of replication from yeast, selectable markers and sequences derived from telomeres and centromere to maintain the stability of the insert during cell division.

    The insert size for plasmids, bacteriophage, PACs, and cosmids is about 0.1-10 kb, 5-25 kb, 100-300 kb, 35-45 kb respectively.
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