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3 April, 00:00

You dilute an original sample 1:30. You then count the number of yeast in the 1:30 dilution. You count an average of 7 cells in the 1/25 mm2 sized boxes. What is the concentration, in cells/ml, of the original sample?

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  1. 3 April, 00:14
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    The concentration, in cells/ml, of the original sample is 52500 cells/ml

    Explanation:

    Given

    - Average number of cells in 1/25 mm^2 sized box (considering 1 box) is 7

    - The depth of each box of a hemocytometer is 0.1 mm

    To find the Volume:

    Volume = 1/25 mm^2 * 0.1 mm^3 = 0.004 mm^3

    Concentration of cells in this volume

    [Cells in the estimated volume] = 7/0.004 mm^3 = 1750 cells/mm^3.

    Knowing 1 mm^3 = 1 ml,

    cell count = 1750 cells/ml.

    Using the dilution factor 1:30 to correct the calculation

    Final cell count after correction = 1750 * 30 = 52500 cells/ml
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