Which neuroanatomical method provides an outline of entire neurons, allowing us to measure cell body size and density?

a. Immunocytochemistry

b. Nissl stain

c. Golgi stain

d. Horseradish peroxidase?

Answer;

B. Nissl stain

Explanation;

-In Nissl staining, cresyl Violet Acetate solution is used to stain Nissl substance in the cytoplasm of neurons in paraformaldehyde or formalin-fixed tissue.

- The neuropil will be stained a granular purple-blue. This stain is commonly used to identify the neuronal structure in brain and spinal cord tissue.

-The Nissl staining method uses basic aniline dye to stain RNA blue, and is used to highlight important structural features of neurons. The Nissl substance appears dark blue due to the staining of ribosomal RNA, giving the cytoplasm a mottled appearance.