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20 February, 20:05

Which of the following statements best describes the termination of transcription in prokaryotes?

A. RNA polymerase transcribes through the polyadenylation signal, causing proteins to associate with the transcript and cut it free from the polymeraseB. RNA polymerase transcribes through the terminator sequence, causing the polymerase to fall off the DNA and release the transcript. C. RNA polymerase transcribes through an intron, and the snRNPs cause the polymerase to let go of the transcript. D. Once transcription has initiated, RNA polymerase transcribes until it reaches the end of the chromosome. E. RNA polymerase transcribes through a stop codon, causing the polymerase to stop advancing through the gene and release the mRNA.

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  1. 20 February, 20:15
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    B. RNA polymerase transcribes through the terminator sequence, causing the polymerase to fall off the DNA and release the transcript.

    Explanation:

    Termination of transcription in prokaryotes can be performed in two ways:

    Rho dependent-Rho factor is a protein that has the ability to bind for RNA polymerase, and to separate it from the template DNA strand Rho independent-there is a specific sequence in DNA rich C and G nucleotides. As RNA polymerase gets to that region, it folds the DNA and creates the hairpin. The hairpin causes the polymerase to fall.
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