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8 October, 20:16

Discuss the quantification of DNA using diphenylamine reaction.

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  1. 8 October, 20:28
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    DNA quantification is necessary to determine the amount of DNA. DNA quantification is important during the various biolmolecular reactions and in case of determining a disease.

    DNA quantification can be done by diphenylamine reaction. The diphenyl amine reacts with the deoxyribose sugar and produce a characteristic blue colir. The absorbance of DNA can be taken at 595 nm and later this absorbance is used for the DNA quantification.
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