The standard potentials for the reduction of nicotinamide adenine dinucleotide (nad+) and oxaloacetate (reactants in the multistep metabolism of glucose) are as follows:

NAD + (aq) + 2H + (aq) + 2e is NAD (aq) + H + (aq) E°=-0.320v

Oxaloacetate (aq) + 2H + (aq) + 2H + (aq) + 2e is malate (aq) E°=-0.166v

formular reaction

E°cell=E electrode - Eanode

If two half reaction, the spontaneeus reaction it will occur if less negative. potential reduction occurs at catode. that is E eclectrode=-0.166v, and Eanode = - 0.320v

To calculate the E°cell

E°cell = - 0.166v - (-0.320v) = 0.154v

Electrical work done and equilibrium constant at suitable is related to each other as

R. T. Ink = n. F. E°cell R, T, K, n, andF they stand for constant gas, absolute temperature, equilibrium, number of electrons and faraday constant respectively the value of

n=2, T as 298K, F as 96500 then K U

R. T. Ink = n. F. E°cell

8.314 J/mol. K*298K, F as 96500C*0.154V

InK=29722/2477.57=162,173.544

K = E11.996