If you want to use PCR technique to amplify markers located on six unlinked locations on the chromosomes, how many total unique primer sequences do you need? A. 1 B. 3 C. 6 D. 8 E. 12

The correct answer is option E, that is, 12.

Explanation:

It is mentioned that the markers are situated in six unlinked sites, the unlinked signifies that they are located on six distinct chromosomes. If one requires to augment the six markers, which are devoid of any definite end sequence, then only six forward primers are adequate. However, if one needs to augment a particular region on the chromosome, then both the reverse and forward primers are needed for each marker. Thus, a sum of 12 primers is required in such a case.

In case, if all the markers are situated in a single chromosome inside a particular region, then only one forward or both reverse and forward primers, that is, two primers would suffice. Generally, the technique of PCR is used to intensify particular fragments and both end and start sequences are illustrated. In such a case, the markers are specified.

Therefore, both reverse and forward primers are needed to augment every marker. Hence, it can be concluded that 12 primers are needed to augment all six markers situated on six unlinked sites.