If two lanes had bands/fragments at the same location could you assume that the DNA at these locations was the same length? Why or why not?

Yes, the DNA was the same length.

Explanation:

Gel electrophoresis is a molecular method that separates charged molecules according to their charge and size across an electric field. DNA is negatively charged hence it moves towards the positive pole.

Due to having the same charge, DNA fragments are separated according to their size or length.

Therefore, Fragments of the same length will move or migrate at the same distance and those having different lengths will move at different distance.