Trixie

Biology

16 July, 01:05

Which of the following statements about PCR is/are FALSE? There may be more than one correct answer.

A) PCR reactions contain DNA template, primers, deoxyribonucleotide triphosphates, and heat - resistant DNA polymerase

B) Each PCR cycle includes three steps: denaturation, annealing, and extension

C) After each PCR cycle, the amount of DNA increases in a linear manner

D) One limitation of PCR is that it requires prior knowledge of the sequence to be amplified

E) PCR is a very sensitive technique and can amplify target sequences present in extremely low copy number

F) The single-stranded DNA primers must anneal to opposite strands of template DNA at distant sites, with their 5′ ends directed toward each other