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10 January, 18:28

The effectiveness of two antibiotics administered together may be greater than the effectiveness of either antibiotic used alone. This is known as synergism seen as evidence of synergism in the disk-diffusion method? What would be zones of inhbiton around each artbote disk that are the same size enhanced zones of inhibition in the area between two adjacent disks no growth of bactena anywhere on the plate O ω a large zone of nhbition around one antbiotic but not around the other O no zone of inhibition in the area between two adjacent disks

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  1. 10 January, 18:51
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    The correct answer is: enhanced zones of inhibition in the area between two adjacent disks.

    Explanation:

    According to the question, the two antibiotics show the property of Synergism. Synergism can be defined as the property of two drugs, where the efficiency of activity (killing the bacteria) obtained on using the two drugs together is far more than the efficiency obtained by using individual drugs separately. In the disk diffusion method, the efficiency in activity (killing of bacteria) of two drugs is determined by plating a nutrient agar media with the particular target bacteria and adding the two antibiotics in two discs separated from each other by a certain distance. Both the antibiotics diffuse into the agar media from the discs and kill the target bacteria. Zone of inhibition can be defined as the circular clear zone on the nutrient agar plate obtained after allowing the bacteria to grow for the required period of time. This is the region where no bacteria grow as they are killed by the antibiotics used. Depending upon the efficiency of the antibiotic used and also its ability to diffuse into the nutrient agar medium, bacteria tends to grow near the periphery of the nutrient agar plate where the antibiotic fails to reach. In the question, as the two antibiotics used show synergistic effect, the region on the nutrient agar plate in between the two discs, where both the antibiotics are able to percolate will show a larger zone of inhibition.
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