What step in glycolysis produces chemical energy that the cell can use?

Answer

Glycolysis is composed of two main steps:

1. Preparatory phase

2. Oxidative phase

In preparatory phase ATP is used for decomposition or conversion of glucose into two 3-carbon molecules i. e. glyceraldehyde 3-phosphate and dihydroxy acetone phosphate.

in oxidative phase, when these molecules convert into two pyruvate, ATP is produced.

Conclusion

Thus chemical energy is produced in oxidative phase of glycolysis.