Aryan Glenn

Biology

14 October, 13:33

You are studying a protein called myprotein in the cytosol. You want to see if another protein called, otherprotein, is required for folding. You isolate the cytosolic fraction using salt precipitation. You want to perform an experiment to find if otherprotein binds and interacts with mylittleprotein. Which assay would be best to perform first?

a. SDS PAGE to see the banding pattern showing all the proteins that interact

b. Immunopreciptation, because this will allow us to pull down proteins that interact

c. Size chromatography, because chances are the proteins that interact are the same size

d. Affinity Chromatography, because we know that it binds other proteins