You decide to clone a gene using a plasmid vector featuring ampicillin resistance and lacz. however, when you try to grow the

e. coli onto a plate containing x-gal and ampicillin, you see no colonies. explain what most likely went wrong during the cloning process.

Question

19 January, 15:36

+3

Answers (1)

Know the Answer?

Skunk · Answer 1

You did not do this experiment in aseptic conditions

You decide to clone a gene using a plasmid vector featuring ampicillin resistance and lacz. however, when you try to grow thee. coli onto a plate containing x-gal and ampicillin, you see no colonies. explain what most likely went wrong during the cloning process.