You have produced antibodies against the GLUT4 glucose transporter and labeled them with a red fluorescent dye. You culture the cells in the absence of insulin and after a 30-minute incubation period, you fix the cells and treat them with the fluorescent antibody. What do you see when you look at the cells in the fluorescence microscope?

Question

Manuel Esparza

Biology

28 October, 18:37

You have produced antibodies against the GLUT4 glucose transporter and labeled them with a red fluorescent dye. You culture the cells in the absence of insulin and after a 30-minute incubation period, you fix the cells and treat them with the fluorescent antibody. What do you see when you look at the cells in the fluorescence microscope?

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Alaina Lindsey · Answer 1

The observation of the fluorescent GLUT4 transporter under a microscope of fluorescence confirms the presence of this transporter in the periphery of fibre and in the region of perinucleus.

Explanation:

The use of red fluorescent dye in labelling of GLUT4 transporter is observed in live cells of mouse. This study showed the predominant presence of GLUT4 in the region of perinucleus and in the fibre periphery. The transporter appeared in small and in large clusters.

The incubation period of 30 minutes and then the glucose ingestion to fix the cell is observed under the view of microscope. This also confirms the constant staining of this protein on the border of the cell of muscle.