A 1.5‑kb fragment of DNA is cloned into a plasmid vector that is 5.5 kb long at the EcoRI site, and the plasmid vector is then used to transform bacteria. If the plasmid DNA is then extracted from a single bacterial colony and digested with EcoRI, what digestion products will be produced if the plasmid contains the fragment?

Question

Yazmin Bowen

Biology

15 September, 09:51

A 1.5‑kb fragment of DNA is cloned into a plasmid vector that is 5.5 kb long at the EcoRI site, and the plasmid vector is then used to transform bacteria. If the plasmid DNA is then extracted from a single bacterial colony and digested with EcoRI, what digestion products will be produced if the plasmid contains the fragment?

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Answers (1)

Know the Answer?

Jayce Novak · Answer 1

The answer is One 1.5‑kb fragment and one 5.5‑kb fragment.

Explanation:

EcoRI digestion will result into 2 fragment corresponding in size to the 1.5‑kb fragment which will be cloned into the plasmid + the 5.5‑kb plasmid itself.