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5 June, 07:13

A common column material used in size exclusion chromatography is dextran, a polysaccharide of glucose. Which type of interaction most likely occurs between proteins and the dextran column material?

A) aromatic

B) hydrophobic

C) salt bridge

D) hydrogen bonding

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  1. 5 June, 07:27
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    D) Hydrogen bonding

    Explanation:

    A hydrogen bond is a kind of electrostatic force of attraction between the hydrogen atom and a more electronegative atom e. g. nitrogen, fluorine, and oxygen.

    It is a weak force of attraction.

    In size-exclusion chromatography, the attraction that exist between the proteins and dextran is hydrogen bonding.

    The several hydroxyl groups thats seen in dextran accounts for why hydrogen bonding exists between dextran column material and the protein.
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