If the primers shown below were used in a DNA analysis of person 1, 2 and 3, what order would the bands appear, from top to bottom, after running the gel?

Question

Taryn Bass

Biology

21 April, 21:15

If the primers shown below were used in a DNA analysis of person 1, 2 and 3, what order would the bands appear, from top to bottom, after running the gel?

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Liliana Ibarra · Answer 1

The order of bands depends on the size of the DNA fragment. When you run the gel, that is carry out gel electrophoresis after doing a PCR, the fragment that is the smallest will travel the most so it will appear at the bottom. The heaviest fragment will have traveled the least so it will be the topmost one because it faces the most resistance in the gel matrix. The faints bands at the very bottom are due to primer dimers.

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