Plaque assays are often used to estimate the number of virions in a sample of a particular volume (the titer). The count is given as plaque-forming units. Which of the following is NOT generally a concern that must be considered in evaluating the results of plaque assays?

Question

Karen Chavez

Biology

23 August, 13:15

Plaque assays are often used to estimate the number of virions in a sample of a particular volume (the titer). The count is given as plaque-forming units. Which of the following is NOT generally a concern that must be considered in evaluating the results of plaque assays?

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Answers (1)

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Tristen Ferrell · Answer 1

The largest problem is that single viruses may create multiple plaques, resulting on overestimates of the number of viruses present.

Explanation:

Plague assay allows to measure the concentration of viruses in a sample, it is a widely used approach for determining the quantity of infectious virus. Therefore the amount of plaques created by a single virus is not a concern when evaluating the results of plaque assays.