This question is based on the experiment that identified the role of the origin of replication using bacteria and a plasmid. The group starts with a plasmid where the oric is removed. What result would we expect to see if we transferred this plasmid into e. Coli?

Question

Kadyn Kirby

Biology

27 October, 06:18

This question is based on the experiment that identified the role of the origin of replication using bacteria and a plasmid. The group starts with a plasmid where the oric is removed. What result would we expect to see if we transferred this plasmid into e. Coli?

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Answers (1)

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Delilah Dickson · Answer 1

It will result in production of a low number of copies desired.

Explanation:

The presence of an appropriate origin of replication in the plasmid to be integrated is for the plasmid to replicate independently of the bacterial cell cycle meaning that a large number of copies are produced per cell. This particular type of replication origin is referred to as "relaxed" as compared with "stringent" which involves plasmid replication with chromosomal replication, resulting in production of low number of copies per cell.