Explain why it is not necessary to continue splicing the gene for human growth hormone into e. Coli once cultures of bacteria with the spliced gene are established

The prokaryote system for transcription does not allow splicing forms of a single gene.

Explanation:

Once the established form of the human growth gene is genetically transformed into the bacteria it is not necessary to consider the splicing of the gene because prokaryote organisms do not possess such transcription mechanism.