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1 August, 18:08

The chain termination method of sequencing:

uses labeled ddNTPs

uses only dideoxynucleotides

uses only deoxynucleotides

uses labeled dNTPs

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  1. 1 August, 18:37
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    Labeled ddNTPs

    Explanation:

    The chain termination method or sanger method consist in 4 synthetis reaction at the same time. Each of them with a different labeled ddNTPs (ddATP, ddCTP, ddGTP and ddTTP) as part of it sythesis sustract. As the reaction goes on, if a ddNTP is added to the new DNA chain, it will stop the elongation becouse DNA polymerase activity depends on the presence of - OH extremes which are miss on ddNTPs.
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