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Braedon Lindsey
Biology
1 September, 20:44
List the steps in isolating embryonic stem cells.
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Simon
1 September, 21:01
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1. Cleavage stage embryo
2. Cultures blastocyst
3. Isolated inner cell mass
4. Irradiated mouse fibroblast feeder cells
5. Cells dissociated and replaced
6. New feeder cells
7. Established embryonic stem cells cultures
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