When the rf of one of the dots observed in a mixture after developing a tlc plate is coincident with that of the reference standard material (rsm), it means:?

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Samantha

Biology

7 September, 02:20

When the rf of one of the dots observed in a mixture after developing a tlc plate is coincident with that of the reference standard material (rsm), it means:?

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Jennings · Answer 1

In thin layer chromatography, when the retention factor (RF) value of one dot observed in a mixture after developing a plate is coincident with the reference standard material, it means that one of the unknown compound is identical to the compound of a known structure or the reference standard material. Therefore, the unknown compound in a mixture was identified using thin layer chromatography.