Select the true statements about SDS‑PAGE, a method of separating proteins. Assume that SDS‑PAGE is performed under reducing conditions. A) Proteins are visualized using a dye that binds to the gel matrix, but not to proteins. B) Sodium dodecyl sulfate binds proteins, resulting in protein‑SDS complexes that are similar in size. C) Protein‑SDS complexes migrate toward the positive electrode. D) SDS‑PAGE utilizes agarose gel to separate proteins. E) Smaller proteins migrate faster through the polyacrylamide gel. F) A protein binds roughly 1.4 times its mass of SDS, resulting in a large overall negative charge.

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Biology

2 October, 13:01

Select the true statements about SDS‑PAGE, a method of separating proteins. Assume that SDS‑PAGE is performed under reducing conditions. A) Proteins are visualized using a dye that binds to the gel matrix, but not to proteins. B) Sodium dodecyl sulfate binds proteins, resulting in protein‑SDS complexes that are similar in size. C) Protein‑SDS complexes migrate toward the positive electrode. D) SDS‑PAGE utilizes agarose gel to separate proteins. E) Smaller proteins migrate faster through the polyacrylamide gel. F) A protein binds roughly 1.4 times its mass of SDS, resulting in a large overall negative charge.

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Cindy Riggs · Answer 1

The correct statements are options C, E and F.

Explanation:

The SDS-PAGE refers to a broadly utilized technique for distinguishing proteins. The SDS or the sodium dodecyl sulfate refers to an ionic detergent. The SDS-protein complexes possess negative charge with identical charge to mass ratio such that when the proteins are run on the gel they are distinguished on the basis of their size.

As the SDS entices a negative charge to the proteins, the migration of the proteins takes place towards the positive electrode. The migration of protein takes place on the basis of the size. Thus, smaller proteins seem to migrate quickly via the gel in comparison to the bigger proteins. The binding of the protein takes place with about 1.4 times its mass of SDS resulting in the formation of a large overall negative charge.