True or False: As discussed in lecture, we use a different assay for each different protein that we purify (For instance, the assay for protein A will be different than the assay for protein B which will be different than the assay for protein C, etc).

Question

Adelyn Vargas

Biology

15 February, 17:15

True or False: As discussed in lecture, we use a different assay for each different protein that we purify (For instance, the assay for protein A will be different than the assay for protein B which will be different than the assay for protein C, etc).

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Answers (1)

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Adyson Owen · Answer 1

The given question is incomplete. The complete question is T/F When performing column chromatography to purify a protein of interest, one needs an assay to identify which fractions that contain the protein of interest. True or False: As discussed in lecture, we use a different assay for each different protein that we purify (For instance, the assay for protein A will be different than the assay for protein B which will be different than the assay for protein C, etc).

Answer: True

Explanation:

The sample contains different types of proteins in it and when the sample into chromatography column.

Different proteins have different affinity and based on the molecular size and weight they are separated.

So, according to the question if some one wants to assay protein A, it will be different for the protein B and different for the assay of protein C. This means that assays for different proteins will be different.

Hence, the given statement is True.