A lab protocol asks you to place 1 gram of tissue into 9 ml of PBS and then homogenize the mixture. You are then asked to take 1 ml of that homogenate and add 9 mls of PBS. Using a standard curve, you determine that the diluted mixture contains 10 micrograms of protein per mL. What is the concentration of protein in the homogenate?

Question

Myles Lyons

Biology

22 April, 07:52

A lab protocol asks you to place 1 gram of tissue into 9 ml of PBS and then homogenize the mixture. You are then asked to take 1 ml of that homogenate and add 9 mls of PBS. Using a standard curve, you determine that the diluted mixture contains 10 micrograms of protein per mL. What is the concentration of protein in the homogenate?

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Answers (1)

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Jayla Christian · Answer 1

100 micrograms per mL.

Explanation:

The PBS diluted mixture used for the standard curve measurement of protein concentration is a 1:10 dilution from the original homogenate of the protein with PBS. So, if the diluted mixture contains 10 micrograms per mL, the original homogenate contains 10x that. 10*10 = 100 micrograms per mL.