Assembly of multi-subunit secretary proteins, such as hemoglobin, occurs within the er and golgi apparatus of a eukaryotic cell. discuss what limitations, if any, exist to the large scale production of genetically engineered hemoglobin from

e. coli.

Genetic engineering often means insertion of a gene and harvest of the protein from huge numbers of bacterial cells.

Hemoglobin, are only functional as an assembly of multiple sub-units. The ability to efficiently harvest the protein of interest and purify it away from all the other proteins normally produced by the host cell, is one of the major limiting factors for the large scale production of genetically engineered hemoglobin.