In a recombinant dna experiment, which enzyme is used to join together dna fragments by forming phosphodiester bonds between nucleotides? recall that this same enzyme joins together okazaki fragments on the lagging strand during dna replication.

Question

Jaydin Collier

Biology

7 May, 13:36

In a recombinant dna experiment, which enzyme is used to join together dna fragments by forming phosphodiester bonds between nucleotides? recall that this same enzyme joins together okazaki fragments on the lagging strand during dna replication.

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Killian Barnett · Answer 1

The correct answer is DNA ligase.

DNA ligase can be described as a DNA-joining enzyme. It has a possibility to link two pieces of DNA if they have the matching ends, using ATP as an energy source. As a result, unbroken molecule of DNA is formed. It is used in recombinant DNA experiments, for example, to insert genes and other pieces of DNA into plasmids.