The PCR technique requires a DNA polymerase from an organism that can endure high heat, such as Thermus aquaticus. What step of the protocol makes the heat-stability most necessary?

Question

Alexander Strickland

Biology

6 January, 10:28

The PCR technique requires a DNA polymerase from an organism that can endure high heat, such as Thermus aquaticus. What step of the protocol makes the heat-stability most necessary?

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Answers (1)

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Skinner · Answer 1

Extension

Explanation:

Polymerase chain reaction is given by Kary Mullis and it contains three steps denaturation, annealing, and extension. In denaturation, the temperature is raised to 96° c so that the DNA can denature.

In annealing, the temperature gets reduced to 55°c so that the primer can bind to the complementary sequence. In extension, Taq polymerase is used to extend the new strand.

This enzyme is isolated from thermophilic bacteria and the temperature here is raised to 72°c so that the enzyme can extend the primer and make the complementary strand. So the right answer is extension.