Which of the following physical methods of microbial control denatures proteins as a mechanism of action?-boiling or autoclaving - incineration - osmotic pressure - radiation

The right answer is - boiling or autoclaving.

In a chemical reaction when the temperature increases, protein denaturation occurs with rates that can be increased. The presence of water promotes denaturation. In a system having a hydrophilic phase and a hydrophobic phase, the denatured proteins under the action of the temperature will be placed at the hydrophilic / hydrophobic interface.