Thirty-six colonies grew in nutrient agar from 0.1 ml of undiluted sample in a standard plate count. How many viable and culturable cells were in the original sample?

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Biology

16 May, 02:57

Thirty-six colonies grew in nutrient agar from 0.1 ml of undiluted sample in a standard plate count. How many viable and culturable cells were in the original sample?

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Tiffany Horne · Answer 1

The sample should obviously be taken from a 10ml source. And since 36 cells grew from a 0.1ml of the sample. Therefore, the cells in the original sample is:

36*10/0.1

=3600 cfu