What is the role of 2'-3'-dideoxynucleoside triphosphates in the chain terminator method of dna sequencing? To generate a series of truncated chains during dna synthesis to serve as primers for dna synthesis to allow detection of the dna fragments by autoradiography to enhance the synthesis of very long dna chains?

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Kirsten Olsen

Biology

20 July, 07:22

What is the role of 2'-3'-dideoxynucleoside triphosphates in the chain terminator method of dna sequencing? To generate a series of truncated chains during dna synthesis to serve as primers for dna synthesis to allow detection of the dna fragments by autoradiography to enhance the synthesis of very long dna chains?

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Camille Joyce · Answer 1

To generate a series of truncated chains during DNA synthesis.

2'-3' dideoxynucleoside triphosphates do not contain 3' hydroxyl group in the sugar ring like dNTPs. Due to this DNA synthesis is stopped as there is no free 3'-OH group to which next dNTPS could be added by DNA polymerase.

This results in the formation of truncated DNA fragments.