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16 February, 03:43

What are the main differences and similarites between the

radioimmunodiffusion (rid) and oucterlony plate technique?

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  1. 16 February, 04:10
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    - Similarities both are diffusion process

    - wells are involved.

    Radial Immunodiffusion (RID) Quantitative test technique quantitative radial immunodiffusion is based upon the reaction between an Ag, and a specific Ab during a diffusion period.

    • Ag placed in a well diffuses into an agar containing the Ag (anti-IgG looking for serum IgG).

    • The Ag-Ab interaction is manifested by well-defined ring of precipitation around the Ag well.

    Explanation:

    While Ouchterlony double diffusion (Immunodiffusion) Qualitative test It is based upon the simultaneous application of Ag and Ab in separate but adjacent wells of an agar plate.

    • As the materials diffuse toward one another, ppt. lines form resulting from the Ag-Ab interactions.

    • If multiple wells of Ag are positioned around an Ab well on the same plate, several patterns of reactivity may be observed.
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