Assume that the conversion of oxidized ubiquinone to reduced ubiquinone by NADH dehydrogenase occurs

on the matrix side of the inner mitochondrial membrane and that its oxidation by cytochrome b-c1 occurs on the intermembrane space side of the membrane (see figures 14-9 and 14-19). What are the consequences of this arrangement for the generation of the H + gradient across the membrane?

The reduction of the oxidized ubiquinone led to the intake of two electrons as well as two protons from water molecules, as shown in Figure 14-19. The protons are further liberated during oxidation. If there is oxidation at one side and reduction at second side of the membrane, There is the movement of one proton for every electron that moves through the membrane. Thus, the movement of electron by the oxidized ubiquinone influences the production of H + gradient.

Explanation:

The reduction of the oxidized ubiquinone led to the intake of two electrons as well as two protons from water molecules, as shown in Figure 14-19. The protons are further liberated during oxidation. If there is oxidation at one side and reduction at the second side of the membrane, There is the movement of one proton for every electron that moves through the membrane. Thus, the movement of an electron by the oxidized ubiquinone influences the production of the H + gradient.