Ligation reactions occur most efficiently between DNA fragments:

a. that have sticky ends.

b. that have blunt ends. that have complementary sticky ends.

c. that are cleaved with different restriction enzymes.

d. where one fragment has a sticky end and the other has a blunt end.

Question

Rich

Biology

25 September, 05:10

Ligation reactions occur most efficiently between DNA fragments:

a. that have sticky ends.

b. that have blunt ends. that have complementary sticky ends.

c. that are cleaved with different restriction enzymes.

d. where one fragment has a sticky end and the other has a blunt end.

+1

Answers (1)

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Roy · Answer 1

B. that have complementary sticky ends

Explanation:

The sealing of nicks between two adjacent residues of a single-strand (ss) break on a double-strand DNA and the joining of double-strand breaks.

This is enzymatically catalyzed by the enzyme DNA ligase.

DNA ligation is the joining ends of 2 DNA molecules by the enzyme, DNA ligase. DNA ligase catalyzes the formation of two covalent phosphodiester bonds between the 3' hydroxyl group of one nucleotides and the 5' phosphate group of another and it is an ATP dependent reaction.

DNA Ligation occur most efficiently between those DNA fragments that have complementary sticky ends.

Ligation reactions occur most efficiently between DNA fragments:a. that have sticky ends.b. that have blunt ends. that have complementary sticky ends.c. that are cleaved with different restriction enzymes.d. where one fragment has a sticky end and the other has a blunt end.

Ligation reactions occur most efficiently between DNA fragments:

a. that have sticky ends.

b. that have blunt ends. that have complementary sticky ends.

c. that are cleaved with different restriction enzymes.

d. where one fragment has a sticky end and the other has a blunt end.